Testis and Epididymis of Indigenous Buffalo Bull: A Biometrical and Histomorphometrical Study

Background and Objective: Testicular and epididymal parameters are important parameters utilized in breeding soundness evaluations in animals. The study was designed to examine the testicular and epididymal biometric and histomorphometric parameters of the indigenous buffalo bulls. Materials and Methods: Biometric values of testis and epididymis of experimental indigenous buffalo bulls (n = 5) were measured using electric balance and vernier-caliper. Histomorphometric values of the testis and epididymis were studied after staining the formalin-fixed tissue sections with routine hematoxylin and eosin stains. Results: The mean length, width and weight of testis were 10.94, 6.32 cm and 178.94 gm and epididymis were 18.88, 1.46 cm and 13.54 gm, respectively. The length and width of epididymis varied in head, body and tail regions. The mean thickness of tunica albuginea was 1503.33 μm and diameter of seminiferous tubules was 211.44 μm. A significant regional difference was observed in epithelial height and epididymal duct diameter among the different gross regions of the epididymis. The epithelial height was the highest in the head and the lowest in the tail region and the reverse was observed in the diameter of epididymal duct. Conclusion: Although the gross and histologic structures of testis and epididymis of indigenous buffalo bulls were almost similar to other domestic animals, values for the biometric and histomorphometric parameters were different from other domestic animals.